Journal: Molecular Medicine Reports
Article Title: Tanshinone IIA improves hypoxic ischemic encephalopathy through TLR-4-mediated NF-κB signal pathway
doi: 10.3892/mmr.2018.9227
Figure Lengend Snippet: Effects of Tan-IIA on inflammatory cytokines in peripheral blood of mice with perinatal HIE. Plasma concentration of (A) IL-1 and (B) TNF-α in peripheral blood of mice with perinatal HIE. Serum levels of (C) CXCL10 and (D) CCL12 in peripheral blood of mice with perinatal HIE. Percent of (E) lymphocytes and (F) monocytes in peripheral blood of mice with perinatal HIE. **P<0.01. Tan-IIA, Tanshinone IIA; HIE, hypoxic ischemic encephalopathy; IL-1, interleukin-1; TNF-α, tumor necrosis factor-α; CXCL10, C-X-C motif chemokine 10; CCL12, chemokine (C-C motif) ligand 12.
Article Snippet: In the protein detection assay, mouse IL-1 (cat. no. MLB00C; R&D Systems, Inc., Minneapolis, MN, USA), mouse IFN-γ (cat. no. MIF00; R&D Systems, Inc., Minneapolis, MN, USA), TNF-α (cat. no. P06804; R&D Systems, Inc.), C-X-C motif chemokine 10 (CXCL10; cat. no. 15945; R&D Systems, Inc.) and chemokine (C-C motif) ligand 12 (CCL12; cat. no. ABIN924766; antibodies-online GmbH, Aachen, Germany) ELISA kits were used to analyze serum levels of inflammatory factors in mice treated with Tan-IIA or PBS.
Techniques: Concentration Assay